Intraerythrocytic Plasmodium falciparum Growth in Serum-Free Medium with an Emphasis on Growth-Promoting Factors
نویسنده
چکیده
Malaria remains a devastating disease, particularly in the tropics. The annual incidence of malaria worldwide is estimated to be between 294 and 500 million clinical cases, while estimates of annual mortality from malaria, caused largely by the protozoan Plasmodium falciparum, range from 0.97 to 2.7 million worldwide (World Malaria Report 20101, World Health Organization; Snow et al., 2005). The emergence of resistance to conventional antimalarial drugs and insecticides means that new chemotherapeutic approaches with alternative targets are needed (Ridley, 2002). Better understandings of antimalarial drugs and the biology of the parasites are needed to allow the development of new medications.
منابع مشابه
Lipid traffic between high density lipoproteins and Plasmodium falciparum-infected red blood cells
Several intraerythrocytic growth cycles of Plasmodium falciparum could be achieved in vitro using a serum free medium supplemented only with a human high density lipoprotein (HDL) fraction (d = 1.063-1.210). The parasitemia obtained was similar to that in standard culture medium containing human serum. The parasite development was incomplete with the low density lipoprotein (LDL) fraction and d...
متن کاملComparative studies of serum-free media and detection techniques for in vitro drug sensitivity assessment of Plasmodium falciparum
Malaria continues to be a devastating disease. In a previous study, we formulated a chemically defined culture medium that is able to sustain the complete intraerythrocytic growth of Plasmodium falciparum. We tested the feasibility of using the medium (CDRPMI) as well as human serum-free media enriched with commercially available human-serum substitutes (GFSRPMI and ALBRPMI) to assess the drug ...
متن کاملIn vitro growth inhibition of Plasmodium falciparum by sera from different regions of the Philippines.
Sera from different malaria endemic regions of the Republic of the Philippines were compared for their ability to inhibit growth of Plasmodium falciparum in vitro. Dialyzed serum was added to synchronous cultures containing schizonts for either the total 48 hr test period or only the last 24 hr in order to analyze the effects on erythrocytic invasion and intraerythrocytic growth, respectively. ...
متن کاملDevelopmental-stage-specific triacylglycerol biosynthesis, degradation and trafficking as lipid bodies in Plasmodium falciparum-infected erythrocytes.
Triacylglycerol (TAG) serves as a major energy storage molecule in eukaryotes. In Plasmodium, however, this established function of TAG appears unlikely, despite detecting previously considerable amount of TAG associated with intraerythrocytic parasites, because plasmodial cells have very little capacity to oxidize fatty acids. Thus, it is plausible that TAG and its biosynthesis in Plasmodium h...
متن کاملImprovement of growth of Plasmodium falciparum fresh clinical isolates by using an established serum-free medium, GIT.
In the present study, we have tried to establish continuous cultures of fresh clinical isolates of P. falciparum by using a serum-free medium, GIT. To examine the ability of GIT to support the parasite growth, the growth of various P. falciparum isolates including two laboratory strains of P. falciparum, FCR3 and K1 was compared in both of GIT and RPMI 1640 medium supplemented by 10% human seru...
متن کامل